A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.
Identifieur interne : 000228 ( Main/Exploration ); précédent : 000227; suivant : 000229A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.
Auteurs : Shengping Shang [République populaire de Chine] ; Bo Wang [République populaire de Chine] ; Song Zhang [République populaire de Chine] ; Guangli Liu [République populaire de Chine] ; Xiaofei Liang [République populaire de Chine] ; Rong Zhang [République populaire de Chine] ; Mark L. Gleason [États-Unis] ; Guangyu Sun [République populaire de Chine]Source :
- Molecular plant pathology [ 1364-3703 ] ; 2020.
Abstract
The ascomycete fungus Colletotrichum fructicola causes diseases on a broad range of plant species. On susceptible cultivars of apple, it induces severe early defoliation and fruit spots, named glomerella leaf spot (GLS), but the mechanisms of pathogenicity have remained elusive. Phytopathogens exhibit small secreted effectors to advance host infection by manipulating host immune reactions. We report the identification and characterization of CfEC92, an effector required for C. fructicola virulence. CfEC92 is a Colletotrichum-specific small secreted protein that suppresses BAX-triggered cell death in Nicotiana benthamiana. Accumulation of the gene transcript was barely detectable in conidia or vegetative hyphae, but was highly up-regulated in appressoria formed during early apple leaf infection. Gene deletion mutants were not affected in vegetative growth, appressorium formation, or appressorium-mediated cellophane penetration. However, the mutants were significantly reduced in virulence toward apple leaves and fruits. Microscopic examination indicated that infection by the deletion mutants elicited elevated deposition of papillae at the penetration sites, and formation of infection vesicles and primary hyphae was retarded. Signal peptide activity, subcellular localization, and cell death-suppressive activity (without signal peptide) assays suggest that CfEC92 could be secreted and perform virulence functions inside plant cells. RNA sequencing and quantitative reverse transcription PCR results confirmed that the deletion mutants triggered elevated host defence reactions. Our results strongly support the interpretation that CfEC92 contributes to C. fructicola virulence as a plant immunity suppressor at the early infection phase.
DOI: 10.1111/mpp.12940
PubMed: 32512647
PubMed Central: PMC7279981
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.</title><author><name sortKey="Shang, Shengping" sort="Shang, Shengping" uniqKey="Shang S" first="Shengping" last="Shang">Shengping Shang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Bo" sort="Wang, Bo" uniqKey="Wang B" first="Bo" last="Wang">Bo Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Song" sort="Zhang, Song" uniqKey="Zhang S" first="Song" last="Zhang">Song Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Guangli" sort="Liu, Guangli" uniqKey="Liu G" first="Guangli" last="Liu">Guangli Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Xiaofei" sort="Liang, Xiaofei" uniqKey="Liang X" first="Xiaofei" last="Liang">Xiaofei Liang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Rong" sort="Zhang, Rong" uniqKey="Zhang R" first="Rong" last="Zhang">Rong Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Gleason, Mark L" sort="Gleason, Mark L" uniqKey="Gleason M" first="Mark L" last="Gleason">Mark L. Gleason</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Sun, Guangyu" sort="Sun, Guangyu" uniqKey="Sun G" first="Guangyu" last="Sun">Guangyu Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32512647</idno><idno type="pmid">32512647</idno><idno type="doi">10.1111/mpp.12940</idno><idno type="pmc">PMC7279981</idno><idno type="wicri:Area/Main/Corpus">000234</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000234</idno><idno type="wicri:Area/Main/Curation">000234</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000234</idno><idno type="wicri:Area/Main/Exploration">000234</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.</title><author><name sortKey="Shang, Shengping" sort="Shang, Shengping" uniqKey="Shang S" first="Shengping" last="Shang">Shengping Shang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Bo" sort="Wang, Bo" uniqKey="Wang B" first="Bo" last="Wang">Bo Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Song" sort="Zhang, Song" uniqKey="Zhang S" first="Song" last="Zhang">Song Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Guangli" sort="Liu, Guangli" uniqKey="Liu G" first="Guangli" last="Liu">Guangli Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Xiaofei" sort="Liang, Xiaofei" uniqKey="Liang X" first="Xiaofei" last="Liang">Xiaofei Liang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Rong" sort="Zhang, Rong" uniqKey="Zhang R" first="Rong" last="Zhang">Rong Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author><author><name sortKey="Gleason, Mark L" sort="Gleason, Mark L" uniqKey="Gleason M" first="Mark L" last="Gleason">Mark L. Gleason</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Sun, Guangyu" sort="Sun, Guangyu" uniqKey="Sun G" first="Guangyu" last="Sun">Guangyu Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant pathology</title><idno type="eISSN">1364-3703</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ascomycete fungus Colletotrichum fructicola causes diseases on a broad range of plant species. On susceptible cultivars of apple, it induces severe early defoliation and fruit spots, named glomerella leaf spot (GLS), but the mechanisms of pathogenicity have remained elusive. Phytopathogens exhibit small secreted effectors to advance host infection by manipulating host immune reactions. We report the identification and characterization of CfEC92, an effector required for C. fructicola virulence. CfEC92 is a Colletotrichum-specific small secreted protein that suppresses BAX-triggered cell death in Nicotiana benthamiana. Accumulation of the gene transcript was barely detectable in conidia or vegetative hyphae, but was highly up-regulated in appressoria formed during early apple leaf infection. Gene deletion mutants were not affected in vegetative growth, appressorium formation, or appressorium-mediated cellophane penetration. However, the mutants were significantly reduced in virulence toward apple leaves and fruits. Microscopic examination indicated that infection by the deletion mutants elicited elevated deposition of papillae at the penetration sites, and formation of infection vesicles and primary hyphae was retarded. Signal peptide activity, subcellular localization, and cell death-suppressive activity (without signal peptide) assays suggest that CfEC92 could be secreted and perform virulence functions inside plant cells. RNA sequencing and quantitative reverse transcription PCR results confirmed that the deletion mutants triggered elevated host defence reactions. Our results strongly support the interpretation that CfEC92 contributes to C. fructicola virulence as a plant immunity suppressor at the early infection phase.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32512647</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>7</Issue><PubDate><Year>2020</Year><Month>07</Month></PubDate></JournalIssue><Title>Molecular plant pathology</Title><ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.</ArticleTitle><Pagination><MedlinePgn>936-950</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/mpp.12940</ELocationID><Abstract><AbstractText>The ascomycete fungus Colletotrichum fructicola causes diseases on a broad range of plant species. On susceptible cultivars of apple, it induces severe early defoliation and fruit spots, named glomerella leaf spot (GLS), but the mechanisms of pathogenicity have remained elusive. Phytopathogens exhibit small secreted effectors to advance host infection by manipulating host immune reactions. We report the identification and characterization of CfEC92, an effector required for C. fructicola virulence. CfEC92 is a Colletotrichum-specific small secreted protein that suppresses BAX-triggered cell death in Nicotiana benthamiana. Accumulation of the gene transcript was barely detectable in conidia or vegetative hyphae, but was highly up-regulated in appressoria formed during early apple leaf infection. Gene deletion mutants were not affected in vegetative growth, appressorium formation, or appressorium-mediated cellophane penetration. However, the mutants were significantly reduced in virulence toward apple leaves and fruits. Microscopic examination indicated that infection by the deletion mutants elicited elevated deposition of papillae at the penetration sites, and formation of infection vesicles and primary hyphae was retarded. Signal peptide activity, subcellular localization, and cell death-suppressive activity (without signal peptide) assays suggest that CfEC92 could be secreted and perform virulence functions inside plant cells. RNA sequencing and quantitative reverse transcription PCR results confirmed that the deletion mutants triggered elevated host defence reactions. Our results strongly support the interpretation that CfEC92 contributes to C. fructicola virulence as a plant immunity suppressor at the early infection phase.</AbstractText><CopyrightInformation>© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shang</LastName><ForeName>Shengping</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-5180-6221</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Song</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Guangli</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Xiaofei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Rong</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gleason</LastName><ForeName>Mark L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Guangyu</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0001-5353-2711</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Plant Pathol</MedlineTA><NlmUniqueID>100954969</NlmUniqueID><ISSNLinking>1364-3703</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Colletotrichum
</Keyword><Keyword MajorTopicYN="Y">apple</Keyword><Keyword MajorTopicYN="Y">effector</Keyword><Keyword MajorTopicYN="Y">host immune response</Keyword><Keyword MajorTopicYN="Y">pathogenicity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32512647</ArticleId><ArticleId IdType="doi">10.1111/mpp.12940</ArticleId><ArticleId IdType="pmc">PMC7279981</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 2013 Aug 16;341(6147):746-51</Citation><ArticleIdList><ArticleId IdType="pubmed">23950531</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2009 Aug;12(4):421-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19608449</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2010 May;28(5):511-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20436464</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2019 Jun;20(6):765-783</Citation><ArticleIdList><ArticleId IdType="pubmed">30945786</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Mar;197(4):1236-49</Citation><ArticleIdList><ArticleId IdType="pubmed">23252678</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(11):e27217</Citation><ArticleIdList><ArticleId IdType="pubmed">22076138</ArticleId></ArticleIdList></Reference><Reference><Citation>Yeast. 1995 Apr 15;11(4):355-60</Citation><ArticleIdList><ArticleId IdType="pubmed">7785336</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomed Res Int. 2013;2013:379850</Citation><ArticleIdList><ArticleId IdType="pubmed">23984355</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Endocrinol. 2008 Feb 13;283(1-2):49-57</Citation><ArticleIdList><ArticleId IdType="pubmed">18177995</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2017 Apr 3;27(7):981-991</Citation><ArticleIdList><ArticleId IdType="pubmed">28318979</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7956-61</Citation><ArticleIdList><ArticleId IdType="pubmed">10393929</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2018 Jan;31(1):101-111</Citation><ArticleIdList><ArticleId IdType="pubmed">29059009</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2012 Oct 18;12(4):484-95</Citation><ArticleIdList><ArticleId IdType="pubmed">23084917</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2017 May;19(5):1717-1729</Citation><ArticleIdList><ArticleId IdType="pubmed">27871149</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2019 Feb 08;10:66</Citation><ArticleIdList><ArticleId IdType="pubmed">30800135</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2016 Sep;17(7):1048-62</Citation><ArticleIdList><ArticleId IdType="pubmed">26619206</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2012 Sep 28;3:222</Citation><ArticleIdList><ArticleId IdType="pubmed">23060891</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2012 Aug;15(4):477-82</Citation><ArticleIdList><ArticleId IdType="pubmed">22658704</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1997 Oct 1;198(1-2):289-96</Citation><ArticleIdList><ArticleId IdType="pubmed">9370294</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2006 Dec;19(12):1420-30</Citation><ArticleIdList><ArticleId IdType="pubmed">17153926</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1999 Dec;11(12):2291-301</Citation><ArticleIdList><ArticleId IdType="pubmed">10590159</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 1999 Nov;83(11):1074</Citation><ArticleIdList><ArticleId IdType="pubmed">30841293</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO Rep. 2018 Jul;19(7):</Citation><ArticleIdList><ArticleId IdType="pubmed">29789386</ArticleId></ArticleIdList></Reference><Reference><Citation>Can J Microbiol. 2003 Jul;49(7):433-42</Citation><ArticleIdList><ArticleId IdType="pubmed">14569284</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2003 Feb;15(2):317-30</Citation><ArticleIdList><ArticleId IdType="pubmed">12566575</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2019 Mar 30;196:120-130</Citation><ArticleIdList><ArticleId IdType="pubmed">29970347</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Immunol. 2004 Feb;16(1):48-62</Citation><ArticleIdList><ArticleId IdType="pubmed">14734110</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Jan 21;10(1):e0115042</Citation><ArticleIdList><ArticleId IdType="pubmed">25606855</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2018 Jul 28;19(1):557</Citation><ArticleIdList><ArticleId IdType="pubmed">30055574</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2009 Jun;4(6):489-92</Citation><ArticleIdList><ArticleId IdType="pubmed">19816126</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Res. 2005;160(2):189-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15881836</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2015 Feb;99(2):249-256</Citation><ArticleIdList><ArticleId IdType="pubmed">30699567</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2014 Jan;98(1):157</Citation><ArticleIdList><ArticleId IdType="pubmed">30708587</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 May 1;25(9):1105-11</Citation><ArticleIdList><ArticleId IdType="pubmed">19289445</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2012 Jun;96(6):912</Citation><ArticleIdList><ArticleId IdType="pubmed">30727399</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2011 Nov;7(11):e1002348</Citation><ArticleIdList><ArticleId IdType="pubmed">22072967</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2014 May 21;26(5):2265-2281</Citation><ArticleIdList><ArticleId IdType="pubmed">24850852</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Apr;46(1):14-33</Citation><ArticleIdList><ArticleId IdType="pubmed">16553893</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 1999 Jul-Aug;27(2-3):186-98</Citation><ArticleIdList><ArticleId IdType="pubmed">10441444</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2012 Aug;15(4):483-92</Citation><ArticleIdList><ArticleId IdType="pubmed">22483402</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2000 Sep;12(9):1579-90</Citation><ArticleIdList><ArticleId IdType="pubmed">11006333</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Feb 24;124(4):803-14</Citation><ArticleIdList><ArticleId IdType="pubmed">16497589</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 2007 Aug;70(8):1278-82</Citation><ArticleIdList><ArticleId IdType="pubmed">17685652</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2018 Jan;31(1):6-12</Citation><ArticleIdList><ArticleId IdType="pubmed">29090656</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2004 Apr;17(4):394-403</Citation><ArticleIdList><ArticleId IdType="pubmed">15077672</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2013 Jan;12(1):2-11</Citation><ArticleIdList><ArticleId IdType="pubmed">22962277</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2008 Apr;20(4):1118-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18390593</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2011 Jun;23(6):2064-86</Citation><ArticleIdList><ArticleId IdType="pubmed">21653195</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2009 Sep;22(9):1069-80</Citation><ArticleIdList><ArticleId IdType="pubmed">19656042</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2012;8(4):e1002643</Citation><ArticleIdList><ArticleId IdType="pubmed">22496661</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Microbiol. 2007 Dec;103(6):2056-64</Citation><ArticleIdList><ArticleId IdType="pubmed">18045389</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2011 Nov;234(5):979-91</Citation><ArticleIdList><ArticleId IdType="pubmed">21691848</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2018 Oct;19(10):2277-2287</Citation><ArticleIdList><ArticleId IdType="pubmed">29745456</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2017;1611:59-73</Citation><ArticleIdList><ArticleId IdType="pubmed">28451972</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2002 Feb 28;415(6875):977-83</Citation><ArticleIdList><ArticleId IdType="pubmed">11875555</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2012 Sep;44(9):1060-5</Citation><ArticleIdList><ArticleId IdType="pubmed">22885923</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Ames (Iowa)</li></settlement><orgName><li>Université d'État de l'Iowa</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Shang, Shengping" sort="Shang, Shengping" uniqKey="Shang S" first="Shengping" last="Shang">Shengping Shang</name></noRegion><name sortKey="Liang, Xiaofei" sort="Liang, Xiaofei" uniqKey="Liang X" first="Xiaofei" last="Liang">Xiaofei Liang</name><name sortKey="Liu, Guangli" sort="Liu, Guangli" uniqKey="Liu G" first="Guangli" last="Liu">Guangli Liu</name><name sortKey="Sun, Guangyu" sort="Sun, Guangyu" uniqKey="Sun G" first="Guangyu" last="Sun">Guangyu Sun</name><name sortKey="Wang, Bo" sort="Wang, Bo" uniqKey="Wang B" first="Bo" last="Wang">Bo Wang</name><name sortKey="Zhang, Rong" sort="Zhang, Rong" uniqKey="Zhang R" first="Rong" last="Zhang">Rong Zhang</name><name sortKey="Zhang, Song" sort="Zhang, Song" uniqKey="Zhang S" first="Song" last="Zhang">Song Zhang</name></country><country name="États-Unis"><region name="Iowa"><name sortKey="Gleason, Mark L" sort="Gleason, Mark L" uniqKey="Gleason M" first="Mark L" last="Gleason">Mark L. Gleason</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantPathoEffV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000228 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000228 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantPathoEffV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32512647
|texte= A novel effector CfEC92 of Colletotrichum fructicola contributes to glomerella leaf spot virulence by suppressing plant defences at the early infection phase.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32512647" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantPathoEffV1
| This area was generated with Dilib version V0.6.38. |  |